Towards the Development of a MagA Reporter Gene Expression Construct for Magnetic Resonance Imaging
نویسنده
چکیده
.............................................................................................................................. ii Acknowledgments .............................................................................................................. iii Table of Contents ............................................................................................................... iv List of Tables ..................................................................................................................... vi List of Figures ................................................................................................................... vii List of Abbreviations ....................................................................................................... viii List of Appendices .............................................................................................................. x Chapter 1 ............................................................................................................................. 1 1 Introduction ................................................................................................................ 1 1.1 Reporter Gene Expression ...................................................................................... 1 1.1.1 Firefly Luciferase ........................................................................................ 3 1.1.2 Reporter Gene Expression in MRI .............................................................. 4 1.1.3 MagA as an MRI Reporter Gene ................................................................ 7 1.2 Nkx2.5 ..................................................................................................................... 9 1.2.1 Sodium Iodide Symporter ......................................................................... 10 1.3 Thesis Objectives .................................................................................................. 15 1.4 References ............................................................................................................. 18 Chapter 2 ........................................................................................................................... 25 2 Induction of the human minimal sodium iodide symporter promoter by RA and Nkx2.5 .......................................................................................................................... 25 2.1 Introduction ........................................................................................................... 25 2.2 Methods ................................................................................................................. 26 2.3 Results ................................................................................................................... 34 2.4 Discussion ............................................................................................................. 40
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